Removal of cadmium(II) from crystallized ferritin.

نویسندگان

  • J Hegenauer
  • P Saltman
  • L Hatlen
چکیده

The cadmium content of crystallized horse spleen ferritin, usually about 2% by weight without special treatment, can be substantially decreased by prolonged dialysis against certain chelating agents, chaotropic ions, or weakly reducing anions. For example, neutral bisulphite buffer (2M) removed 95% of the bound cadmium of crystallization without affecting the iron content, and may thus be valuable for preparing "metal-free" holoferritin for physical-chemical studies.

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عنوان ژورنال:
  • The Biochemical journal

دوره 177 2  شماره 

صفحات  -

تاریخ انتشار 1979